![]() ![]() Following successful sign in, you will be returned to Oxford Academic.Do not use an Oxford Academic personal account. When on the institution site, please use the credentials provided by your institution.Select your institution from the list provided, which will take you to your institution's website to sign in.Click Sign in through your institution.Shibboleth / Open Athens technology is used to provide single sign-on between your institution’s website and Oxford Academic. This authentication occurs automatically, and it is not possible to sign out of an IP authenticated account.Ĭhoose this option to get remote access when outside your institution. Typically, access is provided across an institutional network to a range of IP addresses. If you are a member of an institution with an active account, you may be able to access content in one of the following ways: Get help with access Institutional accessĪccess to content on Oxford Academic is often provided through institutional subscriptions and purchases. ![]() The insulin receptor of the U-937 monocyte-like cell line mirrors the insulin receptor of blood monocytes and target cells it is a useful tool for further in vitro studies of the insulin receptor. Incubation with a phorbol ester tumor promoter, in contrast, inhibits the extent of specific insulin binding to U-937 cells by altering the receptor affinity. The insulin receptor of the U-937 cells can be down-regulated as in the major target cells.Īs in cultured human lymphocytes, preincubation of U-937 cells with prednisolone results in significantly increased insulin binding. Insulin extracted or dissociated spontaneously from the cell surface shows full rebindability to fresh cells. Unlike hepatocytes, these cells do not appear to release extensive insulin-degrading activity and do not degrade cell surface-associated ligand during initial incubations. U-937 cells show a similar affinity for insulin as monocytes. In addition, they internalize the hormone-receptor complex rapidly and extensively at 37 C in a fashion analogous to that of blood monocytes and hepatocytes. We found that the U-937 cells bind insulin specifically and in a time-, temperature-, concentration-, and pH-dependent fashion, similar to circulating monocytes. We, therefore, studied the insulin receptor of a monocyte-like human cell line (U-937) to determine whether it could serve as a model for further investigation of the function of the insulin receptor. Since peripheral monocytes do not divide, they cannot be studied under continuous cell culture conditions. It is important to establish whether the function of the insulin receptor in blood cells reflects that of the major target tissues. ![]() Most clinical studies of the insulin receptor in man have been carried out in circulating cells, mainly monocytes. ![]()
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